Washing Buffer W Tris-Based, 10x stock # AP0502
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W is for Western (blot)
Washing Buffer W Tris-Based, 10x stock
# AP0502
規格介紹:W is for Western (blot)
Washing Buffer W Tris-Based, 10x stock
# AP0502
| Washing Buffer W | AP0502-500 | 500 ml |
Washing Buffer W Tris-Based, 10x stock
# AP0502
A concentrated stock for buffer to rinse and wash the western blot between the procedures without removing blocking and other agents preventing the non-specific reactivity of immunochemicals during the detection procedure. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.
詳細介紹:
Washing Buffer W Tris-Based, 10x stock
# AP0502
A concentrated stock for buffer to rinse and wash the western blot between the procedures without removing blocking and other agents preventing the non-specific reactivity of immunochemicals during the detection procedure. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.
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W is for Western (blot) Stripping Buffer W, ready-to-use # AP0503
Stripping Buffer W, ready-to-use # AP0503 Specially designed for re-probing Western blots, detected with chemiluminiscent and other methods with non-precipitating substrate (i.e. immunofluorescent detection).This formulation effectively and gently removes from blot primary and secondary antibodies, thus allowing to re-probe it again with a new specific antibody.
W is for Western (blot) Antibody Diluent W, ready-to-use # AP0501
Antibody Diluent W, ready-to-use # AP0501 Specifically designed for Western blot and other solid phase immunoassay, such as immune-PCR, dot blot, protein arrays, this buffer prevents non-specific binding of antibody to solid phase and proteins. As a result, using Antibody diluent may dramatically improve the signal/noise ratio and increase the sensitivity of your assay. In our tests we have seen up to 5 times improved sensitivity due to lower background staining. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.
W is for Western (blot) Western Block, ready-to-use # AP0500
Western Block, ready-to-use # AP0500 Specialised solution for blocking free binding sites in Western blots on PVDF, or various types of nitrocellulose membrane. Does not contain large proteins, including serum proteins, BSA, cow milk proteins; low-molecular weight blocking components of this blocking solution do not cause non-specific binding of antibodies to blocking ingredients, while effectively prevent non-specific interactions of test antibody with membrane and transferred proteins on the blot. They also do not prevent (sterically) recognition of the target proteins on the blot due to the size. The buffer is an excellent alternative to self-made skim milk solutions, more effective and improves the signal to noise ratio. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.
Immunohistology buffers Slides Washing Buffer Tris* #AP0476
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Slides Washing Buffer PBS* #AP0474
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Peroxidase Conjugate Diluent #AP0473
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Antibody Diluent-F #AP0475
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Antibody Diluent - P #AP0472
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
