Western Block, ready-to-use # AP0500
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W is for Western (blot)
Western Block, ready-to-use # AP0500
規格介紹:W is for Western (blot)
Western Block, ready-to-use # AP0500
| Western Block, BSA-Free | AP0500-500 | 500 ml |
Western Block, ready-to-use
# AP0500
Specialised solution for blocking free binding sites in Western blots on PVDF, or various types of nitrocellulose membrane. Does not contain large proteins, including serum proteins, BSA, cow milk proteins; low-molecular weight blocking components of this blocking solution do not cause non-specific binding of antibodies to blocking ingredients, while effectively prevent non-specific interactions of test antibody with membrane and transferred proteins on the blot. They also do not prevent (sterically) recognition of the target proteins on the blot due to the size. The buffer is an excellent alternative to self-made skim milk solutions, more effective and improves the signal to noise ratio. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.
詳細介紹:
Western Block, ready-to-use
# AP0500
Specialised solution for blocking free binding sites in Western blots on PVDF, or various types of nitrocellulose membrane. Does not contain large proteins, including serum proteins, BSA, cow milk proteins; low-molecular weight blocking components of this blocking solution do not cause non-specific binding of antibodies to blocking ingredients, while effectively prevent non-specific interactions of test antibody with membrane and transferred proteins on the blot. They also do not prevent (sterically) recognition of the target proteins on the blot due to the size. The buffer is an excellent alternative to self-made skim milk solutions, more effective and improves the signal to noise ratio. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.
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Immunohistology buffers Slides Washing Buffer Tris* #AP0476
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Slides Washing Buffer PBS* #AP0474
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Peroxidase Conjugate Diluent #AP0473
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Antibody Diluent-F #AP0475
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Antibody Diluent - P #AP0472
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Immunohistology buffers Section Block #AP0471
Immunohistology buffers Low background and strong specific reaction. Standardized staining. ISO 9008 certified for diagnostics A B Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from: non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often "affinity purified" means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions) low specificity antibodies among specific ones in polyclonal fragments of fallen apart IgGs in stored preparations, including monoclonal separate heavy and light chains of specific antibodies, produced by most hybridomas All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even higher, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making many domains accessible that are charged and capable of binding the test immunoglobulins in a non-specific manner. The standard means to block non-specific binding of specific antibody preparation is to add an irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of the specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays isntead contain short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.(fugure on the left, bottom) I'm a paragraph. Click here to add your own text and edit me. I’m a great place for you to tell a story and let your users know a little more about you. Aptum's collection of Immunohistology buffers has also some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.
Retriever-Grade High Adhesivity Tissue Slides Histobond+F, Routine & Cryostat
General Technical Characteristics the slides are made of soda-lime glass of third hydrolytic class. This glass fulfils all relevant optical requirements for light microscopy; meet the requirements of DIN ISO 8037/1; dimensions: approx. 76 x 26 mm; thickness: approx. 1 mm (tol. ± 0.05 mm); pre-cleaned and ready for use. Our slides are cleaned thoroughly by multi-stage processes without using any surfactants. Thus we accomplish ready for use, pre-cleaned, well wettable slides‘ surfaces; autoclavable finely ground edges with 90º shape. We apply water-cooled grinding processes which results in remarkably smooth and burr-free edges. have an imprinted frosted end of approx. 20 mm, which can be printed with various printer systems and labelled with permanent markers. Dark markings contrast, especially well with the bright colours of the labelling areas and thus facilitate the identification of preparations. The thin layer of the labelling area prevents the slides from sticking together, which facilitates the use of these slides on automated machinery. supplied in plastic boxes of 100 pieces, 20 boxes in a carton Histobond+F, Routine & Cryostat #AP0603 High quality tissue slidesfor cryostat tissues and routine IHC staining for pathology and histology experiments.
Retriever-Grade High Adhesivity Tissue Slides Histobond+X, Covalent Binding
General Technical Characteristics the slides are made of soda-lime glass of third hydrolytic class. This glass fulfils all relevant optical requirements for light microscopy; meet the requirements of DIN ISO 8037/1; dimensions: approx. 76 x 26 mm; thickness: approx. 1 mm (tol. ± 0.05 mm); pre-cleaned and ready for use. Our slides are cleaned thoroughly by multi-stage processes without using any surfactants. Thus we accomplish ready for use, pre-cleaned, well wettable slides‘ surfaces; autoclavable finely ground edges with 90º shape. We apply water-cooled grinding processes which results in remarkably smooth and burr-free edges. have an imprinted frosted end of approx. 20 mm, which can be printed with various printer systems and labelled with permanent markers. Dark markings contrast, especially well with the bright colours of the labelling areas and thus facilitate the identification of preparations. The thin layer of the labelling area prevents the slides from sticking together, which facilitates the use of these slides on automated machinery. supplied in plastic boxes of 100 pieces, 20 boxes in a carton Histobond+X, Covalent Binding #AP0602 Superior quality slides with coating covalently binding the tissue. Use for all small tissue section (or small at least in one dimention). Surerior for fat tissues, arrays, and similar experiments with valuable materials.
