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Histobond+R
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Retriever-Grade High Adhesivity Tissue Slides
Histobond+R, Supercharged
規格介紹:Retriever-Grade High Adhesivity Tissue Slides
Histobond+R, Supercharged
HistoBond®+R | AP0601-001 | 1 Box x100 |
AP0601-020 | 20 Boxes of 100 |
General Technical Characteristics
the slides are made of soda-lime glass of third hydrolytic class. This glass fulfils all relevant optical requirements for light microscopy;
meet the requirements of DIN ISO 8037/1;
dimensions: approx. 76 x 26 mm;
thickness: approx. 1 mm (tol. ± 0.05 mm);
pre-cleaned and ready for use. Our slides are cleaned thoroughly by multi-stage processes without using any surfactants. Thus we accomplish ready for use, pre-cleaned, well wettable slides‘ surfaces;
autoclavable
finely ground edges with 90º shape. We apply water-cooled grinding processes which results in remarkably smooth and burr-free edges.
have an imprinted frosted end of approx. 20 mm, which can be printed with various printer systems and labelled with permanent markers. Dark markings contrast, especially well with the bright colours of the labelling areas and thus facilitate the identification of preparations. The thin layer of the labelling area prevents the slides from sticking together, which facilitates the use of these slides on automated machinery.
supplied in plastic boxes of 100 pieces, 20 boxes in a carton
Histobond+R, Supercharged
#AP0601
Tissue slides with superior charged surface, consistency of lots, and best physical characteristics of glass used,
Recommended for all impirtant experiments, experimental pathology, IHC and IF.
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H-Recovery, 10x
The type of epitope defines the most appropriate recovery buffer As mentioned above, most of currently used commercial antibodies are derived either against a bacterially expressed fragment of target protein or a synthetic peptide epitope. A standard approach used for development of a specific antibody usually means that a well-exposed surface epitope area and/or unique fragment of a protein were chosen for antibody development. However, when an antibody is prepared against a unique mutated or modified epitope, or unique epitope that differs in closely related proteins of the same family of molecules, then the choice of the epitope is rather limited, and it may be not exposed or be a part of a structure which is present only in a mature, folded molecule. In those cases, R-Universal may be not the best buffer, as well as standard EDTA etc. The chart below may be used to choose the most appropriate buffer for epitope recovery in relation to the type of the epitope: This epitope is usually recognised by antibodies raised against a hydrophilic peptide or bacterially produced fragments of a target protein. Discontinuous epitope requires at least some proper folding of the molecule. Antibodies to such epitopes are usually raised against native antigen, often glycosylated, or in complex with other proteins. Structural epitope requires proper folding of the native protein, usually with cysteine bridges being intact. In mature protein such epitope is usually hidden. Usually antibody is raised against an immunogenicbut not highly hydrophilic peptide epitope.. In total we offer 5 different buffers for antigen recovery. Most of epitopes can be perfectly recovered in R-Universal buffer, including those usually recovered in Citrate/EDTA/Tris-EDTA buffers. Two new buffers offer solutions for rare, but still existing cases when antibody gives an incorrect pattern on a retrieved section or no staining at all. We also now offer analogues of standard Low (Citrate) and High (EDTA) pH-buffers in case you already use antibodies known to work after tissues being processed in one of these buffers. Below we provide another chart that helps you to choose the most appropriate buffer even for antibodies that were never tested in FF/PE sections. Alternatively, use the Epitope Recovery Buffer Kit to establish the best buffer in test antigen unmasking.L-Recovery, 10x
The type of epitope defines the most appropriate recovery buffer As mentioned above, most of currently used commercial antibodies are derived either against a bacterially expressed fragment of target protein or a synthetic peptide epitope. A standard approach used for development of a specific antibody usually means that a well-exposed surface epitope area and/or unique fragment of a protein were chosen for antibody development. However, when an antibody is prepared against a unique mutated or modified epitope, or unique epitope that differs in closely related proteins of the same family of molecules, then the choice of the epitope is rather limited, and it may be not exposed or be a part of a structure which is present only in a mature, folded molecule. In those cases, R-Universal may be not the best buffer, as well as standard EDTA etc. The chart below may be used to choose the most appropriate buffer for epitope recovery in relation to the type of the epitope: This epitope is usually recognised by antibodies raised against a hydrophilic peptide or bacterially produced fragments of a target protein. Discontinuous epitope requires at least some proper folding of the molecule. Antibodies to such epitopes are usually raised against native antigen, often glycosylated, or in complex with other proteins. Structural epitope requires proper folding of the native protein, usually with cysteine bridges being intact. In mature protein such epitope is usually hidden. Usually antibody is raised against an immunogenicbut not highly hydrophilic peptide epitope.. In total we offer 5 different buffers for antigen recovery. Most of epitopes can be perfectly recovered in R-Universal buffer, including those usually recovered in Citrate/EDTA/Tris-EDTA buffers. Two new buffers offer solutions for rare, but still existing cases when antibody gives an incorrect pattern on a retrieved section or no staining at all. We also now offer analogues of standard Low (Citrate) and High (EDTA) pH-buffers in case you already use antibodies known to work after tissues being processed in one of these buffers. Below we provide another chart that helps you to choose the most appropriate buffer even for antibodies that were never tested in FF/PE sections. Alternatively, use the Epitope Recovery Buffer Kit to establish the best buffer in test antigen unmasking.U-Recovery, 10x
The type of epitope defines the most appropriate recovery buffer As mentioned above, most of currently used commercial antibodies are derived either against a bacterially expressed fragment of target protein or a synthetic peptide epitope. A standard approach used for development of a specific antibody usually means that a well-exposed surface epitope area and/or unique fragment of a protein were chosen for antibody development. However, when an antibody is prepared against a unique mutated or modified epitope, or unique epitope that differs in closely related proteins of the same family of molecules, then the choice of the epitope is rather limited, and it may be not exposed or be a part of a structure which is present only in a mature, folded molecule. In those cases, R-Universal may be not the best buffer, as well as standard EDTA etc. The chart below may be used to choose the most appropriate buffer for epitope recovery in relation to the type of the epitope: This epitope is usually recognised by antibodies raised against a hydrophilic peptide or bacterially produced fragments of a target protein. Discontinuous epitope requires at least some proper folding of the molecule. Antibodies to such epitopes are usually raised against native antigen, often glycosylated, or in complex with other proteins. Structural epitope requires proper folding of the native protein, usually with cysteine bridges being intact. In mature protein such epitope is usually hidden. Usually antibody is raised against an immunogenicbut not highly hydrophilic peptide epitope.. In total we offer 5 different buffers for antigen recovery. Most of epitopes can be perfectly recovered in R-Universal buffer, including those usually recovered in Citrate/EDTA/Tris-EDTA buffers. Two new buffers offer solutions for rare, but still existing cases when antibody gives an incorrect pattern on a retrieved section or no staining at all. We also now offer analogues of standard Low (Citrate) and High (EDTA) pH-buffers in case you already use antibodies known to work after tissues being processed in one of these buffers. Below we provide another chart that helps you to choose the most appropriate buffer even for antibodies that were never tested in FF/PE sections. Alternatively, use the Epitope Recovery Buffer Kit to establish the best buffer in test antigen unmasking.T-Universal,10x
The type of epitope defines the most appropriate recovery buffer As mentioned above, most of currently used commercial antibodies are derived either against a bacterially expressed fragment of target protein or a synthetic peptide epitope. A standard approach used for development of a specific antibody usually means that a well-exposed surface epitope area and/or unique fragment of a protein were chosen for antibody development. However, when an antibody is prepared against a unique mutated or modified epitope, or unique epitope that differs in closely related proteins of the same family of molecules, then the choice of the epitope is rather limited, and it may be not exposed or be a part of a structure which is present only in a mature, folded molecule. In those cases, R-Universal may be not the best buffer, as well as standard EDTA etc. The chart below may be used to choose the most appropriate buffer for epitope recovery in relation to the type of the epitope: This epitope is usually recognised by antibodies raised against a hydrophilic peptide or bacterially produced fragments of a target protein. Discontinuous epitope requires at least some proper folding of the molecule. Antibodies to such epitopes are usually raised against native antigen, often glycosylated, or in complex with other proteins. Structural epitope requires proper folding of the native protein, usually with cysteine bridges being intact. In mature protein such epitope is usually hidden. Usually antibody is raised against an immunogenicbut not highly hydrophilic peptide epitope.. In total we offer 5 different buffers for antigen recovery. Most of epitopes can be perfectly recovered in R-Universal buffer, including those usually recovered in Citrate/EDTA/Tris-EDTA buffers. Two new buffers offer solutions for rare, but still existing cases when antibody gives an incorrect pattern on a retrieved section or no staining at all. We also now offer analogues of standard Low (Citrate) and High (EDTA) pH-buffers in case you already use antibodies known to work after tissues being processed in one of these buffers. Below we provide another chart that helps you to choose the most appropriate buffer even for antibodies that were never tested in FF/PE sections. Alternatively, use the Epitope Recovery Buffer Kit to establish the best buffer in test antigen unmasking.R-UNIVERSAL Epitope Recovery Buffer (10x stock)
One for All: the Universal Buffer We proudly present our novel, patent-pending R-Universal (R stands for Retriever) buffer for antigen unmasking/epitope recovery on formalin-fixed, paraffin embedded sections[1]. Being specifically developed for 2100 Retriever, it was thoroughly tested in pathology labs across the UK, and is now available for all users of Retriever and other researchers who work with routine tissue material. Why the Universal Buffer? Epitope recovery on formalin-fixed, paraffin embedded tissue sections, requires heatinduced treatment in buffer or, sometimes, proteolytic treatment of the deparaffinized tissue section. Which buffer to use, greatly depends on the exact antibody and the properties of the recognized epitope, therefore, one can find in literature and practice use of many buffers, including Citrate pH 3.4, Citrate 6.0, EDTA 8.0, Tris 9.0- 10.0, TrisEDTA, etc. Moreover, for individual antigens also the time of recovery in the individual buffer should be defined, as this may be different, and the treatment often destroys the epitope. Aptum offers a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups. The buffer was extensively tested in pathology Departments in United Kingdom, and has shown excellent results when used with different antibodies, including those that normally require for successful staining treatment only in Low, or only High pH buffers, or require protolithic treatment. Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in 2100 Retriever (www.antigen-retriever.com). Using Universal Buffer in Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections. Properties Clear, non-toxic solution. May be slightly irritable for sensitive skin and eyes. Please wear gloves while handling the 10x stock. Presentation R-Universal Buffer is supplied as 10x concentrate. For epitope recovery dilute 1 part of stock with 9 parts of deionized water. Application Place deparaffinized sections into slide chamber, fill the buffer until the frosted part of the glass (50 to 70 ml of ready buffer for a chamber, depends on the load). Run a recovery cycle in 2100 Retriever according to its Manual. We recommend using 2100 Retriever, as the most accurately controlling refolding of the protein. If you use another system, please calibrate the time required for processing; it may depend on your unit temperature and pressure. Stability and Storage The preparation is stable for 1 year when stored unopened at +40C. Every lot is issued with a certificate indicating the expiry date. After opening, store at +40C in the refrigerator and use within 6 months. Certification Each lot is certified for compliance to specifications. The product is produced under DIN EN ISO 9001 :2008 Quality Management system for the products in Immunoassay Development and Measurement, Products for Bioanalytics and lmmunoassays.The Unique Retriever Cat. No. 62700-10
Our Unique Retriever solves the problem of staining formalin fixed tissues. This is an affordable solution to all known major problems with immunohistochemistry on paraffin sections. Ease of use combined with high reproducibility of the results will give you the best quality immunostaining. The Retriever is a bench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining. The model has been designed to ensure identical processing of all the samples during a processing cycle, as well as the identical processing of the samples in individual sessions. The retriever preserves processed tissues. Now you can: Run antigen unmasking in 6 various buffers at once. Perform gentle antigen retrieval that does not damage the tissue morphology. Get identical results every time. Compare series of slides treated in independent sessions. How does the Retriever work? Ok, basically it is a pressure cooker. However, a pressure cooker specially designed to unmask the antigen on tissue sections. The core principle is heating of the chambers with the slides at high temperature (>120°C) and high pressure. Sensors control the heating profile for the temperature and pressure to be reached at certain pace and over certain time. We did a lot of tests to find the optimal settings. When the required temperature is reached, it will be kept for several minutes. After that the slides will be cooling over 2 hours. Specially designed thermal walls of the unit control the speed of cooling of the inner chamber and slides. Who would benefit from using our Retriever? Investigative Pathology, where the high quality of staining (a picture may be published!) is required. Any labs thatis short on technical personnel: any student or post-doc can process slides for the staining without using much time Small routine pathology labs, where a limited number of slides should be processed daily Anyone who uses highly valuable samples, such as tissue arrays or unique tissue samples: Simplicity and reliability of the unit ensures the safety of your sample, and a high quality antigen unmasking 62700-10 Retriever 115 volt each 62705-01 Slide Chamber 3/pk 62705-02 Chamber Rack each 62705-03 Lifting Device each 62705-06 Cord Set (US) each 62705-07 Green Silicone Sealing Gasket each塑膠玻片儲存盒Fisherbrand™ Slide Holders 產品編號:FS-12-588-20
Description Index tabs on each holder for slide reference and classification Each holder accepts four slides, sizes 3 × 1 in. or 75 × 25mm Supplied with or without plastic filing box Slide holder set includes 12 5 × 3 in. (127 × 76mm) slide holders Holders can also be stored in the Fisherbrand Microslide Cabinet (Cat. No. 12-588-30) Specifications Device Holds Four microscope slides per card (75 x 25mm and 3 x 1 inch slides of any thickness) Length (Metric) 127 mm (Slide Holder) Size 75 x 25 mm and 3 x 1 inch slides of any thickness Material Cardboard fitted with a protective plastic frame Width (Metric) 76 mm (Slide Holder) Width (English) 3 in. (Slide Holder) Includes File box Length (Metric) Slide 75 mm Length (English) 12.5 in. (Slide Holder) Width (English) Slide 1 in. Length (English) Slide 3 in. Width (Metric) Slide 25 mmFisherbrand™ Securline™ Marker II Pens 產品編號:FS-14-905-30(Black)/35(Red)
Description Specially designed for marking Superfrost™ and frosted microslides and plastic embedding cassettes Performs well on ceramic, vinyl, paper, porcelain, metal, Mylar™ and other types of glass and plastic. Please test on material before using. Ink is resistant to many normal laboratory solutions when used in procedures that do not require abrasive contact Resists a variety of such solvents as alcohol, acetone and xylene Perfect for histology and cytology work Ink will withstand steam or gas sterilization and temperatures from cryogenic to approximately 150°C (238°F) Line width is approximately 0.015 in. Ink dries to the touch almost immediately, but should be allowed to fix for five minutes before processing in solvent baths Black or red Specifications Product Type Securline™ Marker II Pen Width (Metric) Line 0.38mm Color Black Quantity 10 Pack For Use With (Application) Used for marking Superfrost™ and frosted microslides and Plastic embedding cassettes